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Protocols

Cucurbit chlorotic yellows virus (CCYV)

Abrahamian, P. E., Seblani, R., Sobh, H., and Abou-Jawdah, Y. 2013. Detection and quantitation of two cucurbit criniviruses in mixed infection by real-time RT-PCR. Journal of Virological Methods 193:320-326.  

Jailani, K. A. A., Hendricks, K., Roberts, D. P., and Paret, M. L. 2021. Development of a simple one-step multiplex RT-PCR system for simultaneous detection of DNA and RNA viruses of Cucurbit leaf crumple virus, Cucurbit yellow stunting disorder virus, Squash vein yellowing virus, and Cucurbit chlorotic yellows virus. Physiological and Molecular Plant Pathology 116:101734.

Kubota, K., Usugi, T., and Tsuda, S. 2011. Production of antiserum and immunodetection of cucurbit chlorotic yellows virus, a novel whitefly-transmitted crinivirus. Journal of General Plant Pathology 77:116-120. 

Okuda, M., Okuda, S., and Iwai, H.  2015. Detection of Cucurbit chlorotic yellows virus from Bemisia tabaci captured on sticky traps using reverse transcription loop-mediated isothermal amplification (RT-LAMP) and simple template preparation. Journal of Virological Methods 221:9-14. 

Wang, Z. Y., Gu, Q. S., Sun, B. J., Sun, H., Li, H. L., Liang, X. Z., Yuan, Y., Liu, R. L., and Shi, Y. 2014. One-step reverse transcription loop mediated isothermal amplification assay for sensitive and rapid detection of cucurbit chlorotic yellows virus. Journal of Virological Methods 195:63-66. 

Zang, L., Qiao, N., Sun, X., Zhang, X., Zhao, D., Li, J., and Zhu, X. 2022. Reverse transcription recombinase polymerase amplification assay for rapid detection of the cucurbit chlorotic yellows virus. Journal of Virological Methods 300:114388.

Cucumber green mottle mosaic virus (CGMMV)

Hasiów-Jaroszewska, B.,  Budzyńska, D., Borodynko-Filas, N. 2019. Genetic diversity of the cucumber green mottle mosaic virus and the development of RT-LAMP assay for its detection. Plant Protection Science 55(1):1-7.

Hongyun, C., Wenjun, Z., Qinsheng, G., Qing, C., Shiming, L., and Shuifang, Z. 2008. Real time TaqMan RT-PCR assay for the detection of Cucumber green mottle mosaic virus. Journal of Virological Methods 149(2):326-329.

Kwon, S. J., Cho, Y. E., Kim, M. H., and Seo, J. K. 2021. A one-step reverse-transcription loop-mediated isothermal amplification assay optimized for the direct detection of cucumber green mottle mosaic virus in cucurbit seeds. Molecular and Cellular Probes 60:101775.

Li, J., Wei, Q., Liu,Y.,  Tan, X., Zhang, W., Wu, J., Charimbu, M.K., Hu, B.,  Cheng, Z., Yu, C., and Tao, X. 2013. One-step reverse transcription loop-mediated isothermal amplification for the rapid detection of cucumber green mottle mosaic virus. Journal of Virological Methods 193:583-588.

Pitman, T. L., Vu, S., Tian, T., Posis, K., and Falk, B. W. 2022. Genome and phylogenetic analysis of cucumber green mottle mosaic virus global isolates and validation of a highly sensitive RT-qPCR assay. Plant Disease 106(6):1713-1722.

Sui, X., Li, R., Shamimuzzaman, M., Wu, Z., and Ling, K.-S. 2019. Understanding the transmissibility of cucumber green mottle mosaic virus in watermelon seeds and seed health assays. Plant Disease 103:1126-1131.

Cucurbit leaf crumple virus (CuLCrV)

Brown, J. K., Idris, A. M., Olsen, M. W., Miller, M. E., Isakeit, T., and Anciso, J. 2000. Cucurbit leaf curl virus, a new whitefly transmitted Geminivirus in Arizona, Texas, and Mexico. Plant Disease 84(7):809.

Guzman, P., Sudarshana, M. R., Seo, Y.-S., Rojas, M. R., Natwick, E., Turini, T., Mayberry, K., and Gilbertson, R. L. 2000. A new bipartite geminivirus (Begomovirus) causing leaf curl and crumpling in cucurbits in the Imperial Valley of California. Plant Disease 84(4):488.

Hagen C., Rojas M. R., Sudarshana, M. R., Xoconostle-Cazares B., Natwick, E. T., Turini, T. A., Gilbertson, R. L. 2008. Biology and molecular characterization of Cucurbit leaf crumple virus, an emergent cucurbit-infecting Begomovirus in the Imperial Valley of California. Plant Disease 92(5):781-793.

Jailani, K. A. A., Hendricks, K., Roberts, D. P., and Paret, M. L. 2021. Development of a simple one-step multiplex RT-PCR system for simultaneous detection of DNA and RNA viruses of Cucurbit leaf crumple virus, Cucurbit yellow stunting disorder virus, Squash vein yellowing virus, and Cucurbit chlorotic yellows virus. Physiological and Molecular Plant Pathology 116:101734.

Cucurbit yellow stunting disorder virus (CYSDV)

Abrahamian, P. E., Seblani, R., Sobh, H., and Abou-Jawdah, Y. 2013. Detection and quantitation of two cucurbit criniviruses in mixed infection by real-time RT-PCR. Journal of Virological Methods 193:320-326.

Berdiales, B., Bernal, J. J., Sáez, E., Woudt, B., Beitia, F., and Rodríguez-Cerezo, E. 1999. Occurrence of Cucurbit yellow stunting disorder virus (CYSDV) and beet pseudo-yellows virus in cucurbit crops in Spain and transmission of CYSDV by two biotypes of Bemisia tabaci. European Journal of Plant Pathology 105:211-215.

Célix, A., López-Sesé, A., Almarza, N., Gómez-Guillamón, M. L., and Rodríguez-Cerezo, E. 1996. Characterization of cucurbit yellow stunting disorder virus, a Bemisia tabaci-transmitted closterovirus. Phytopathology 86:1370-1376.

Cotillon, A.C., Desbiez, C., Bouyer, S., Wipf-Scheibel, C., Gros, C., Delecolle, B., and Lecoq, H. Production of a polyclonal antiserum against the coat protein of Cucurbit yellow stunting disorder crinivirus expressed in Escherichia coli. EPPO Bulletin 35(1):99-103.

Gil-Salas, F. M., Morris, J., Colyer, A., Budge, G., Boonham, N., Cuadrado, I. M., and Janssen, D. 2007. Development of real-time RT-PCR assays for the detection of Cucumber vein yellowing virus (CVYV) and Cucurbit yellow stunting disorder virus (CYSDV) in the whitefly vector Bemisia tabaci. Journal of Virological Methods 146:45-51.

Hourani, H. and Abou-Jawdah, Y. 2003. Immunodiagnosis of Cucurbit yellow stunting disorder virus using polyclonal antibodies developed against recombinant coat protein. Journal Plant Pathology 85(3):197-204.

Jailani, K. A. A., Hendricks, K., Roberts, D. P., and Paret, L. M. 2021. Development of a simple one-step multiplex RT-PCR system for simultaneous detection of DNA and RNA viruses of cucurbit leaf crumple virus, cucurbit yellow stunting disorder virus, squash vein yellowing virus, and cucurbit chlorotic yellows virus. Physiological and Molecular Plant Pathology 116:101734.

Mondal, S., Jenkins Hladky, L., and Wintermantel, W. M. 2023. Differential seasonal prevalence of yellowing viruses infecting melon crops in southern California and Arizona determined by multiplex RT-PCR and RT-qPCR. Plant Disease in press.

Papayiannis, L. C., Hunter, S. C., Iacovides, T., and Brown, J. K. 2010. Detection of Cucurbit yellow stunting disorder virus in cucurbit leaves using sap extracts and real-time TaqMan reverse transcription (RT) polymerase chain reaction (PCR). Journal of Phytopathology 158:487-495.

Rubio, L., Soong, J., Kao, J., and Falk, B. W. 1999. Geographic distribution and molecular variation of isolates of three whitefly-borne closteroviruses of cucurbits: Lettuce infectious yellows virus, Cucurbit yellow stunting disorder virus, and Beet pseudo-yellows virus. Phytopathology 89:707–711.

Ruiz, L., Janssen, D., Velasco, L., Segundo, E., and Cuadrado, I. M. 2002. Quantitation of cucurbit yellow stunting disorder virus in Bemisia tabaci (Genn.) using digoxigenin-labelled hybridisation probes. Journal of Virological Methods 101(1-2):95-103.

Tian, T., Klaassen, V. A., Soong, J., Wisler, G., Duffus, J. E., and Falk, B. W. 1996. Generation of cDNAs specific to lettuce infectious yellows closterovirus and other whitefly-transmitted viruses by RT-PCR and degenerate oligonucleotide primers corresponding to the closterovirus gene encoding the heat shock protein 70 homolog. Phytopathology 86:1167-1173.

Wintermantel, W. M. and Hladky, L. L. 2010. Methods for detection and differentiation of existing and new crinivirus species through multiplex and degenerate primer RT-PCR. Journal of Virological Methods 170:106-114.

Squash leaf curl China virus (SLCCNV)

Venkataravanappa, V., Kumar, H. D. V., Nandan, M., Hiremath, S., Shankarappa, K. S., Krishna, Reddy, M., and Lakshminarayana Reddy, C. N. 2022. Characterization of Squash leaf curl China virus associated with mosaic and vein clearing disease of pointed gourd (Trichosanthes dioica Roxb.) and its vector whitefly cryptic species. Indian Phytopathology 75:573-581.

Venkataravanappa, V., Reddy, C. N. L., Nandan, M., Hiremath, S., Ashwathappa, K. V., Shankarappa, K. S., Vinay Kumar, H. D., and Reddy, M. K. 2021. Transmission, characterization and occurrence of recombination in Indian strain of squash leaf curl China virus associated with yellow mosaic and leaf curl disease of summer squash. 3 Biotech 11(6):265.

Wu, H., Li, M., Hong, N., Peng, B., and Gu, Q. 2020. Molecular and biological characterization of melon-infecting squash leaf curl China virus in China. Journal of Integrative Agriculture 19:570-577.

Watermelon crinkle leaf-associated virus 1 and 2 (WCLaV-1 and WCLaV-2)

Adeleke, I. A., Kavalappara, S. R., Tolerance, T., Bennett, J., McGregor, C., Srinivasan, R., and Bag, S. 2022. First report of watermelon crinkle leaf-associated virus 1 naturally infecting watermelon (Citrullus lanatus) in Georgia, United States. Plant Disease 106(8):2273.

Hendricks, K. E. M., Hernandez, R. N., Roberts, P. D., Isakeit, T., and Alabi, O. J. 2022. First report of watermelon crinkle leaf-associated virus 1 (WCLaV-1) and WCLaV-2 in watermelon (Citrullus lanatus) plants coinfected with cucurbit chlorotic yellows virus in Florida. Plant Disease 106(1):339.

Hernandez, R. N., Isakeit, T., Al Rwahnih, M., Villegas, C., and Alabi, O. J. 2021. First report of watermelon crinkle leaf-associated virus 1 (WCLaV-1) and WCLaV-2 infecting watermelon (Citrullus lanatus) in the United States. Plant Disease 105:2025.

Maeda, M. H. K., Koyama, L. H. H., Campos, R. N. S., Kauffmann, C. M., Souza, J. O., Gilbertson, R., Inoue-Nagata, A. K., Freitas, D. M. S., Nogueira, D. R. S., Melo, F. L., and Nagata, T. 2022. First report of watermelon crinkle leaf-associated virus 1 and 2 infecting watermelon (Citrullus lanatus) plants in Brazil. Plant Disease 106:773.

Xin, M., Cao, M., Liu, W., Ren, Y., Zhou, X., and Wang, X. 2017. Two negative-strand RNA viruses identified in watermelon represent a novel clade in the order Bunyavirales. Frontiers in Microbiology 8:1514.

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